A simple and sensitive spectrofluorimetric method has been developed for estimation of Levofloxacin in tablet dosage form. The piperazinyl moiety in Levofloxacin acts as fluorophore. Levofloxacin produces strong fluorescence at 455 nm (λem) with excitation at 289 nm (λex) in water. Linearity range was found to be 50-300 ng/ml with mean recovery 100.53%. The correlation co-efficient was found to be 0.9994. The limit of detection (LOD) and limit of quantification (LOQ) for the developed method were found to be 2.33 and 7.07 ng/ml, respectively. The content of Levofloxacin in two marketed formulations was found to be 99.44% and 99.77% of labelled amount. The developed method was found to be specific, sensitive, accurate and precise. It was successfully used for estimation of Levofloxacin in its tablet dosage form.
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